Karahoda, Betim and Pardeshi, Lakhansing and Ulas, Mevlut and Dong, Zhiqiang and Shirgaonkar, Niranjan and Guo, Shuhui and Wang, Fang and Tan, Kaeling and Sarikaya-Bayram, Özlem and Bauer, Ingo and Dowling, Paul and Fleming, Alastair B. and Pfannenstiel, Brandon T. and Luciano-Rosario, Dianiris and Berger, Harald and Graessle, Stefan and Alhussain, Mohamed M. and Strauss, Joseph and Keller, Nancy P. and Wong, Koon Ho and Bayram, Ozgur (2022) The KdmB-EcoA-RpdA-SntB chromatin complex binds regulatory genes and coordinates fungal development with mycotoxin synthesis. Nucleic Acids Research, 50 (17). pp. 9797-9813. ISSN 0305-1048

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Abstract

Chromatin complexes control a vast number of epigenetic developmental processes. Filamentous fungi present an important clade of microbes with poor understanding of underlying epigenetic mechanisms. Here, we describe a chromatin binding complex in the fungus Aspergillus nidulans composing of a H3K4 histone demethylase KdmB, a cohesin acetyltransferase (EcoA), a histone deacetylase (RpdA) and a histone reader/E3 ligase protein (SntB). In vitro and in vivo evidence demonstrate that this KERS complex is assembled from the EcoA-KdmB and SntB-RpdA heterodimers. KdmB and SntB play opposing roles in regulating the cellular levels and stability of EcoA, as KdmB prevents SntB-mediated degradation of EcoA. The KERS complex is recruited to transcription initiation start sites at active core promoters exerting promoter-specific transcriptional effects. Interestingly, deletion of any one of the KERS subunits results in a common negative effect on morphogenesis and production of secondary metabolites, molecules important for niche securement in filamentous fungi. Consequently, the entire mycotoxin sterigmatocystin gene cluster is downregulated and asexual development is reduced in the four KERS mutants. The elucidation of the recruitment of epigenetic regulators to chromatin via the KERS complex provides the first mechanistic, chromatin-based understanding of how development is connected with small molecule synthesis in fungi.

Item Type:	Article
Keywords:	Gene Regulation; Chromatin; Epigenetics;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	17020
Identification Number:	https://doi.org/10.1093/nar/gkac744
Depositing User:	Ozgur Bayram
Date Deposited:	13 Mar 2023 14:26
Journal or Publication Title:	Nucleic Acids Research
Publisher:	Oxford University Press
Refereed:	Yes
URI:	https://academic.oup.com/nar
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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