Margalit, Anatte and Sheehan, David and Carolan, James C. and Kavanagh, Kevin
(2022)
Exposure to the Pseudomonas aeruginosa secretome alters the proteome and secondary metabolite production of Aspergillus fumigatus.
Microbiology, 168 (3).
ISSN 1350-0872
Abstract
The fungal pathogen Aspergillus fumigatus is frequently cultured from the sputum of cystic fibrosis (CF) patients along with the
bacterium Pseudomonas aeruginosa. A. fumigatus secretes a range of secondary metabolites, and one of these, gliotoxin, has
inhibitory effects on the host immune response. The effect of P. aeruginosa culture filtrate (CuF) on fungal growth and gliotoxin
production was investigated. Exposure of A. fumigatus hyphae to P. aeruginosa cells induced increased production of gliotoxin
and a decrease in fungal growth. In contrast, exposure of A. fumigatus hyphae to P. aeruginosa CuF led to increased growth and
decreased gliotoxin production. Quantitative proteomic analysis was used to characterize the proteomic response of A. fumigatus upon exposure to P. aeruginosa CuF. Changes in the profile of proteins involved in secondary metabolite biosynthesis (e.g.
gliotoxin, fumagillin, pseurotin A), and changes to the abundance of proteins involved in oxidative stress (e.g. formate dehydrogenase) and detoxification (e.g. thioredoxin reductase) were observed, indicating that the bacterial secretome had a profound
effect on the fungal proteome. Alterations in the abundance of proteins involved in detoxification and oxidative stress highlight
the ability of A. fumigatus to differentially regulate protein synthesis in response to environmental stresses imposed by competitors such as P. aeruginosa. Such responses may ultimately have serious detrimental effects on the host.
| Item Type: |
Article
|
| Keywords: |
Exposure; Pseudomonas aeruginosa; secretome;
proteome; secondary metabolite production;
Aspergillus fumigatus; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
17026 |
| Identification Number: |
https://doi.org/10.1099/mic.0.001164 |
| Depositing User: |
James Carolan
|
| Date Deposited: |
13 Mar 2023 16:13 |
| Journal or Publication Title: |
Microbiology |
| Publisher: |
Microbiology Society |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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