Freitag, Michael and Sarikaya Bayram, Özlem and Bayram, Ozgur and Karahoda, Betim and Meister, Cindy and Köhler, Anna M. and Thieme, Sabine and Elramli, Nadia and Frawley, Dean and McGowan, Jamie and Fitzpatrick, David A. and Schmitt, Kerstin and de Assis, Leandro Jose and Valerius, Oliver and Goldman, Gustavo H. and Braus, Gerhard H. (2022) F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans. PLOS Genetics, 18 (12). e1010502. ISSN 1553-7404

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Abstract

Fungal growth and development are coordinated with specific secondary metabolism. This coordination requires 8 of 74 F-box proteins of the filamentous fungus Aspergillus nidulans. F-box proteins recognize primed substrates for ubiquitination by Skp1-Cul1-Fbx (SCF) E3 ubiquitin RING ligases and degradation by the 26S proteasome. 24 F-box proteins are found in the nuclear fraction as part of SCFs during vegetative growth. 43 F-box proteins interact with SCF proteins during growth, development or stress. 45 F-box proteins are associated with more than 700 proteins that have mainly regulatory roles. This corroborates that accurate surveillance of protein stability is prerequisite for organizing multicellular fungal development. Fbx23 combines subcellular location and protein stability control, illustrating the complexity of F-box mediated regulation during fungal development. Fbx23 interacts with epigenetic methyltransferase VipC which interacts with fungal NF-κB-like velvet domain regulator VeA that coordinates fungal development with secondary metabolism. Fbx23 prevents nuclear accumulation of methyltransferase VipC during early development. These results suggest that in addition to their role in protein degradation, F-box proteins also control subcellular accumulations of key regulatory proteins for fungal development.

Item Type:	Article
Keywords:	F-box receptor; mediated control; substrate stability; subcellular; location; cellular development; Aspergillus nidulans;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	17046
Identification Number:	https://doi.org/10.1371/journal.pgen.1010502
Depositing User:	David Fitzpatrick
Date Deposited:	20 Mar 2023 16:17
Journal or Publication Title:	PLOS Genetics
Publisher:	Public Library of Science
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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