MURAL - Maynooth University Research Archive Library

    Healthy versus inflamed lung environments differentially affect mesenchymal stromal cells

    Rolandsson Enes, Sara and Hampton, Thomas H. and Barua, Jayita and McKenna, David H. and dos Santos, Claudia C. and Amiel, Eyal and Ashare, Alix and Liu, Kathleen D. and Krasnodembskaya, Anna D. and English, Karen and Stanton, Bruce A. and Rocco, Patricia R.M. and Matthay, Michael A. and Weiss, Daniel J. (2021) Healthy versus inflamed lung environments differentially affect mesenchymal stromal cells. European Respiratory Journal, 58 (200414). pp. 1-14. ISSN 0903-1936

    Download (1MB) | Preview

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    Background Despite increased interest in mesenchymal stromal cell (MSC)-based cell therapies for acute respiratory distress syndrome (ARDS), clinical investigations have not yet been successful and our understanding of the potential in vivo mechanisms of MSC actions in ARDS remains limited. ARDS is driven by an acute severe innate immune dysregulation, often characterised by inflammation, coagulation and cell injury. How this inflammatory microenvironment influences MSC functions remains to be determined. Aim The aim of this study was to comparatively assess how the inflammatory environment present in ARDS lungs versus the lung environment present in healthy volunteers alters MSC behaviour. Methods Clinical-grade human bone marrow-derived MSCs (hMSCs) were exposed to bronchoalveolar lavage fluid (BALF) samples obtained from ARDS patients or from healthy volunteers. Following exposure, hMSCs and their conditioned media were evaluated for a broad panel of relevant properties, including viability, levels of expression of inflammatory cytokines, gene expression, cell surface human leukocyte antigen expression, and activation of coagulation and complement pathways. Results Pro-inflammatory, pro-coagulant and major histocompatibility complex (self-recognition) related gene expression was markedly upregulated in hMSCs exposed ex vivo to BALF obtained from healthy volunteers. These changes were less apparent and often opposite in hMSCs exposed to ARDS BALF samples. Conclusion These data provide new insights into how hMSCs behave in healthy versus inflamed lung environments, and strongly suggest that the inflamed environment in ARDS induces hMSC responses that are potentially beneficial for cell survival and actions. This further highlights the need to understand how different disease environments affect hMSC functions.

    Item Type: Article
    Keywords: Healthy; versus; inflamed; lung; environments; differentially affect; mesenchymal stromal cells;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Human Health Institute
    Item ID: 17116
    Identification Number:
    Depositing User: Karen English
    Date Deposited: 24 Apr 2023 14:43
    Journal or Publication Title: European Respiratory Journal
    Publisher: European Respiratory Society
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads