Yao, Yong-Gang and de Vries, Paul S. and Sabater-Lleal, Maria and Chasman, Daniel I. and Trompet, Stella and Ahluwalia, Tarunveer S. and Teumer, Alexander and Kleber, Marcus E. and Chen, Ming-Huei and Wang, Jie Jin and Attia, John R. and Marioni, Riccardo E. and Steri, Maristella and Weng, Lu-Chen and Pool, Rene and Grossmann, Vera and Brody, Jennifer A. and Venturini, Cristina and Tanaka, Toshiko and Rose, Lynda M. and Oldmeadow, Christopher and Mazur, Johanna and Basu, Saonli and Frånberg, Mattias and Yang, Qiong and Ligthart, Symen and Hottenga, Jouke J. and Rumley, Ann and Mulas, Antonella and de Craen, Anton J. M. and Grotevendt, Anne and Taylor, Kent D. and Delgado, Graciela E. and Kifley, Annette and Lopez, Lorna M. and Berentzen, Tina L. and Mangino, Massimo and Bandinelli, Stefania and Morrison, Alanna C. and Hamsten, Anders and Tofler, Geoffrey and de Maat, Moniek P. M. and Draisma, Harmen H. M. and Lowe, Gordon D. and Zoledziewska, Magdalena and Sattar, Naveed and Lackner, Karl J. and Völker, Uwe and McKnight, Barbara and Huang, Jie and Holliday, Elizabeth G. and McEvoy, Mark A. and Starr, John M. and Hysi, Pirro G. and Hernandez, Dena G. and Guan, Weihua and Rivadeneira, Fernando and McArdle, Wendy L. and Slagboom, P. Eline and Zeller, Tanja and Psaty, Bruce M. and Uitterlinden, André G. and de Geus, Eco J. C. and Stott, David J. and Binder, Harald and Hofman, Albert and Franco, Oscar H. and Rotter, Jerome I. and Ferrucci, Luigi and Spector, Tim D. and Deary, Ian J. and März, Winfried and Greinacher, Andreas and Wild, Philipp S. and Cucca, Francesco and Boomsma, Dorret I. and Watkins, Hugh and Tang, Weihong and Ridker, Paul M. and Jukema, Jan W. and Scott, Rodney J. and Mitchell, Paul and Hansen, Torben and O'Donnell, Christopher J. and Smith, Nicholas L. and Strachan, David P. and Dehghan, Abbas (2017) Comparison of HapMap and 1000 Genomes Reference Panels in a Large-Scale Genome-Wide Association Study. PLoS ONE, 12 (1). pp. 1-22. ISSN 1932-6203

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Abstract

An increasing number of genome-wide association (GWA) studies are now using the higher resolution 1000 Genomes Project reference panel (1000G) for imputation, with the expectation that 1000G imputation will lead to the discovery of additional associated loci when compared to HapMap imputation. In order to assess the improvement of 1000G over HapMap imputation in identifying associated loci, we compared the results of GWA studies of circulating fibrinogen based on the two reference panels. Using both HapMap and 1000G imputation we performed a meta-analysis of 22 studies comprising the same 91,953 individuals. We identified six additional signals using 1000G imputation, while 29 loci were associated using both HapMap and 1000G imputation. One locus identified using HapMap imputation was not significant using 1000G imputation. The genome-wide significance threshold of 5×10−8 is based on the number of independent statistical tests using HapMap imputation, and 1000G imputation may lead to further independent tests that should be corrected for. When using a stricter Bonferroni correction for the 1000G GWA study (P-value < 2.5×10−8), the number of loci significant only using HapMap imputation increased to 4 while the number of loci significant only using 1000G decreased to 5. In conclusion, 1000G imputation enabled the identification of 20% more loci than HapMap imputation, although the advantage of 1000G imputation became less clear when a stricter Bonferroni correction was used. More generally, our results provide insights that are applicable to the implementation of other dense reference panels that are under development.

Item Type:	Article
Keywords:	Comparison; HapMap; 1000 Genomes; Reference Panels; Large-Scale; Genome-Wide; Association Study;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	17194
Identification Number:	https://doi.org/10.1371/journal.pone.0167742
Depositing User:	Lorna Lopez
Date Deposited:	17 May 2023 09:52
Journal or Publication Title:	PLoS ONE
Publisher:	Public Library of Science
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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