Tierney, Ciara and Bazou, Despina and Lê, Giao and Dowling, Paul and O'Gorman, Peter (2021) Saliva-omics in plasma cell disorders- Proof of concept and potential as a non-invasive tool for monitoring disease burden. Journal of Proteomics, 231. p. 104015. ISSN 1874-3919

Preview

Download (1MB) | Preview

Abstract

Multiple Myeloma (MM), the second most common lymphoid cancer worldwide, is characterised by the uninhibited proliferation of terminally differentiated Blymphocytes. Leading to The diagnosis typically requires the presence of a monoclonal protein (M protein) and the demonstration of CRAB features (hypercalcemia, renal impairment, anaemia and bone lesions). MM is considered incurable as, due to serial clonal evolution, the vast majority of patients succumb to treatment refractory disease. MGUS (Monoclonal Gammopathy of Unknown Uncertain Significance) is the pre-malignant form of MM and, although 93% of MM patients exhibit M protein production associated with MGUS before diagnosis, little is known about the switch from pre-malignant to malignant disease. To explore this disease transition further, LC-MS/MS analysis was carried out to identify potential salivary biomarkers to monitor disease burden. FABP5 was detected in saliva as having a significant increase in abundance when MGUS was compared to symptomatic MM. The levels of FABP5 decreased after treatment indicating correlation with tumour burden. This finding was validated using western blot analysis and ELISA analysis. Significance: The field of biomarker discovery has focused largely on serum as a biofluid. Saliva is a readily available biofluid that, as a biomarker resource, has been relatively un-explored. The identification of changes in saliva indicating disease progression underlines the utility of saliva as a non-invasive source of informative biomarkers reflecting disease burden and progression.

Item Type:	Article
Additional Information:	Cite as: Ciara Tierney, Despina Bazou, Giao Lê, Paul Dowling, Peter O'Gorman, Saliva-omics in plasma cell disorders- Proof of concept and potential as a non-invasive tool for monitoring disease burden, Journal of Proteomics, Volume 231, 2021, 104015, ISSN 1874-3919, https://doi.org/10.1016/j.jprot.2020.104015.
Keywords:	biomarker-discovery; Multiple Myeloma; Saliva-omics; lymphoid cancer;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	17261
Identification Number:	https://doi.org/10.1016/j.jprot.2020.104015
Depositing User:	Paul Dowling
Date Deposited:	01 Jun 2023 10:16
Journal or Publication Title:	Journal of Proteomics
Publisher:	Elsevier
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

Repository Staff Only(login required)

Item control page

Downloads

Origin of downloads