Ali, Asghar and Hasan, Phool and Irfan, Mohammad and Uddin, Amad and Khan, Ashba and Saraswat, Juhi and Maguire, Ronan and Kavanagh, Kevin and Patel, Rajan and Joshi, Mukesh C. and Azam, Amir and Mohsin, Mohd. and Haque, Qazi Mohd. Rizwanul and Abid, Mohammad (2021) Development of Oxadiazole-Sulfonamide-Based Compounds as Potential Antibacterial Agents. ACS Omega, 6 (42). pp. 27798-27813. ISSN 2470-1343

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Abstract

In this work, substituted 1,2,4-oxadiazoles (OX1− OX27) were screened against five bacterial strains, identified to be OX7 and OX11 as growth inhibitors with minimum inhibitory concentration (MIC) values of 31.25 and 15.75 μg/mL, respectively. The growth inhibitory property of OX7 and OX11 was further validated by disk diffusion, growth curve, and time kill curve assays. Both disrupted biofilm formation with 92−100% reduction examined by the XTT assay were further visualized by scanning electron microscopy analysis. These compounds in combination with ciprofloxacin also exhibit synergy against Escherichia coli cells. With insignificant cytotoxic behavior on HEK293 cells, human red blood cells, and Galleria mellonella larvae, OX11 was tested against 28 multidrug resistant environmental isolates of bacteria and showed inhibition of Kluyvera georgiana and Citrobacter werkmanii strains with 32 and 16 μg/mL MIC values, respectively. The synergistic behavior of OX11 with ampicillin showed many fold reductions in MIC values against K. georgiana and Klebsiella pneumoniae multidrug resistant strains. Further, transmission electron microscopy analysis of OX11-treated E. coli cells showed a significantly damaged cell wall, which resulted in the loss of integrity and cytosolic oozing. OX11 showed significant changes in the secondary structure of human serum albumin (HSA) in the presence of OX11, enhancing HSA stability. Overall, the study provided a suitable core for further synthetic alterations and development as an antibacterial agent

Item Type:	Article
Additional Information:	Cite as: Ali, A., Hasan, P., Irfan, M., Uddin, A., Khan, A., Saraswat, J., Maguire, R., Kavanagh, K., Patel, R., Joshi, M.C., Azam, A., Mohsin, M., Haque, Q.M.R. & Abid, M. 2021, "Development of Oxadiazole-Sulfonamide-Based Compounds as Potential Antibacterial Agents", ACS omega, vol. 6, no. 42, pp. 27798-27813.
Keywords:	Antimicrobial agents;Assays;Bacteria;Biofilms;Toxicological synergy
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	17323
Identification Number:	https://doi.org/10.1021/acsomega.1c03379
Depositing User:	Dr. Kevin Kavanagh
Date Deposited:	15 Jun 2023 09:40
Journal or Publication Title:	ACS Omega
Publisher:	ACS Publications
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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