Miricescu, Alexandra and Brazel, Ailbhe Jane and Beegan, Joseph and Wellmer, Frank and Graciet, Emmanuelle (2023) Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response. Plant Direct, 7 (8). ISSN 2475-4455

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Abstract

Waterlogging leads to major crop losses globally, particularly for waterlogging-sensitive crops such as barley. Waterlogging reduces oxygen availability and results in additional stresses, leading to the activation of hypoxia and stress response pathways that promote plant survival. Although certain barley varieties have been shown to be more tolerant to waterlogging than others and some tolerance-related quantitative trait loci have been identified, the molecular mechanisms underlying this trait are mostly unknown. Transcriptomics approaches can provide very valuable information for our understanding of waterlogging tolerance. Here, we surveyed 21 barley varieties for the differential transcriptional activation of conserved hypoxia-response genes under waterlogging and selected five varieties with different levels of induction of core hypoxia-response genes. We further characterized their phenotypic response to waterlogging in terms of shoot and root traits. RNA sequencing to evaluate the genome-wide transcriptional responses to waterlogging of these selected varieties led to the identification of a set of 98 waterlogging-response genes common to the different datasets. Many of these genes are orthologs of the so-called “core hypoxia response genes,” thus highlighting the conservation of plant responses to waterlogging. Hierarchical clustering analysis also identified groups of genes with intrinsic differential expression between varieties prior to waterlogging stress. These genes could constitute interesting candidates to study “predisposition” to waterlogging tolerance or sensitivity in barley.

Item Type:	Article
Additional Information:	Cite as: Miricescu, A., Brazel, A.J., Beegan, J., Wellmer, F. & Graciet, E. 2023, "Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response", Plant direct, vol. 7, no. 8, pp. e518-n/a
Keywords:	barley; Hordeum vulgare; RNA‐seq; transcriptomics; waterlogging;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	17742
Identification Number:	https://doi.org/10.1002/pld3.518
Depositing User:	Ailbhe Brazel
Date Deposited:	26 Oct 2023 13:18
Journal or Publication Title:	Plant Direct
Publisher:	John Wiley & Sons, Inc
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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