Alves de Castro, Patrícia and Figueiredo Pinzan, Camila and dos Reis, Thaila Fernanda and Valero, Clara and Van Rhijn, Norman and Menegatti, Carla and de Freitas Migliorini, Ivan Lucas and Bromley, Michael and Fleming, Alastair B. and Traynor, Aimee M. and Sarikaya-Bayram, Özlem and Bayram, Ozgur and Malavazi, Iran and Ebel, Frank and Barbosa, Júlio César Jerônimo and Fill, Taícia and Pupo, Monica Tallarico and Goldman, Gustavo H. (2024) Aspergillus fumigatus mitogen-activated protein kinase MpkA is involved in gliotoxin production and self-protection. Nature Communications, 15 (1). pp. 1-18. ISSN 2041-1723

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Abstract

Aspergillus fumigatus is a saprophytic fungus that can cause a variety of human diseases known as aspergillosis. Mycotoxin gliotoxin (GT) production is important for its virulence and must be tightly regulated to avoid excess production and toxicity to the fungus. GT self-protection by GliT oxidoreductase and GtmA methyltransferase activities is related to the subcellular localization of these enzymes and how GT can be sequestered from the cytoplasm to avoid increased cell damage. Here, we show that GliT:GFP and GtmA:GFP are localized in the cytoplasm and in vacuoles during GT production. The Mitogen-Activated Protein kinase MpkA is essential for GT production and self-protection, interacts physically with GliT and GtmA and it is necessary for their regulation and subsequent presence in the vacuoles. The sensor histidine kinase SlnASln1 is important for modulation of MpkA phosphorylation. Our work emphasizes the importance of MpkA and compartmentalization of cellular events for GT production and self-defense.

Item Type:	Article
Keywords:	Aspergillus fumigatus; mitogen-activated protein; kinase; MpkA; gliotoxin production self-protection;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	19185
Identification Number:	https://doi.org/10.1038/s41467-023-44329-1
Depositing User:	Ozgur Bayram
Date Deposited:	14 Nov 2024 15:43
Journal or Publication Title:	Nature Communications
Publisher:	Nature Publshing Group
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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