Luciano-Rosario, Dianiris and Barda, Omer and Tannous, Joanna and Frawley, Dean and Bayram, Ozgur and Prusky, Dov and Sionov, Edward and Keller, Nancy P.
(2023)
The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum.
Fungal Genetics and Biology, 169 (103837).
pp. 1-10.
ISSN 1087-1845
Abstract
Epigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacetylase. Deletion of kdmB phenocopied some but not all characteristics of the ΔsntB mutant. KdmB deletion strains exhibited reduced lesion development on Golden Delicious apples and this was accompanied by decreased production of patulin and citrinin in host tissue. In addition, ΔkdmB mutants were sensitive to several cell wall stressors which possibly contributed to the decreased virulence observed on apples. Slight differences in spore production and germination rates of ΔkdmB mutants in vitro did not impact overall diameter growth in culture.
| Item Type: |
Article
|
| Keywords: |
Epigenetics;
Virulence;
Secondary metabolism;
Fungal biology;
Post-harvest disease;
Mycotoxin; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
19187 |
| Identification Number: |
https://doi.org/10.1016/j.fgb.2023.103837 |
| Depositing User: |
Ozgur Bayram
|
| Date Deposited: |
14 Nov 2024 16:16 |
| Journal or Publication Title: |
Fungal Genetics and Biology |
| Publisher: |
Elsevier Inc. |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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