Reiber, Kathrin and Reeves, Emer P. and Neville, Claire M. and Winkler, Robert and Gebhardt, Peter and Kavanagh, Kevin and Doyle, Sean (2005) The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron. FEMS Microbiology Letters, 248 (1). pp. 83-91. ISSN 0378-1097

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Abstract

Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD (200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy.

Item Type:	Article
Keywords:	NRPS; Iron; Siderophore; MALDI ToF; Secondary metabolites; Proteomics; 40-PPTase;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	231
Identification Number:	https://doi.org/10.1016/j.femsle.2005.05.028
Depositing User:	Dr. Sean Doyle
Date Deposited:	14 Jul 2005
Journal or Publication Title:	FEMS Microbiology Letters
Publisher:	Elsevier B.V.
Refereed:	Yes
Funders:	Higher Education Authority (HEA)
URI:	http://femsle.oxfordjournals.org
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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