Carberry, Stephen and Neville, Claire M. and Kavanagh, Kevin and Doyle, Sean (2006) Analysis of major intracellular proteins of Aspergillus fumigatus by MALDI mass spectrometry: Identification and characterisation of an elongation factor 1B protein with glutathione transferase activity. Biochemical and Biophysical Research Communications, 341 (4). pp. 1096-1104. ISSN 0006-291X

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Abstract

Aspergillus fumigatus is a recognised human pathogen, especially in immunocompromised individuals. The availability of the annotated A. fumigatus genome sequence will significantly accelerate our understanding of this organism. However, limited information is available with respect to the A. fumigatus proteome. Here, both a direct proteomic approach (2D-PAGE and MALDI-MS) and a sub-proteomic strategy involving initial glutathione affinity chromatography have been deployed to identify 54 proteins from A. fumigatus primarily involved in energy metabolism and protein biosynthesis. Furthermore, two novel eukaryotic elongation factor proteins (eEF1Bc), termed ElfA and B have been identified and phylogenetically confirmed to belong to the eEF1Bc class of GST-like proteins. One of these proteins (ElfA) has been purified to homogeneity, identified as a monomeric enzyme (molecular mass = 20 kDa; pI = 5.9 and 6.5), and found to exhibit glutathione transferase activity specific activities (mean ± standard deviation, n = 3) of 3.13 ± 0.27 and 3.43 ± 1.0 lmol/min/mg, using CDNB and ethacrynic acid, respectively. Overall, these data highlight the importance of new approaches to dissect the proteome of, and elucidate novel functions within, A. fumigatus. 2006 Elsevier Inc. All rights reserved.

Item Type:	Article
Keywords:	Aspergillosis; Proteomics; 2D-PAGE; Oxidative stress; Protein synthesis; GST; Glutathione;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	321
Identification Number:	https://doi.org/10.1016/j.bbrc.2006.01.078
Depositing User:	Dr. Sean Doyle
Date Deposited:	15 Jun 2006
Journal or Publication Title:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Refereed:	Yes
Funders:	Higher Education Authority (HEA), National Institute of Allergy and Infectious Disease, Health Research Board (HRB)
URI:	http://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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