Renwick, Julie and Reeves, Emer P. and Wientjes, Frans B. and Kavanagh, Kevin
(2006)
Translocation of proteins homologous to human neutrophil
p47phox and p67phox to the cell membrane in activated
hemocytes of Galleria mellonella.
Developmental and Comparative Immunology.
Abstract
Activation of the superoxide forming respiratory burst oxidase of human neutrophils, crucial in host defence, requires the cytosolic proteins p47phox and p67phox which translocate to the plasma membrane upon cell stimulation and activate flavocytochrome b558, the redox centre of this enzyme system. We have previously demonstrated the presence of proteins (67 and 47 kDa) in hemocytes of the insect Galleria mellonella homologous to proteins of the superoxide-forming NADPH oxidase complex of neutrophils. The work presented here illustrates for the first time translocation of homologous hemocyte proteins, 67 and 47 kDa from the cytosol to the plasma membrane upon phorbol 12-myristate 13 acetate (PMA)activation. In hemocytes, gliotoxin (GT), the fungal secondary metabolite significantly suppressed PMA-induced superoxide generation in a concentration dependent manner and reduced translocation to basel nonstimulated levels.
Primarily these results correlate translocation of hemocyte 47 and 67 kDa proteins with PMA induced oxidase activity.
Collectively results presented here, demonstrate further cellular and functional similarities between phagocytes of insects and mammals and further justify the use of insects in place of mammals for modelling the innate immune response to microbial pathogens.
| Item Type: |
Article
|
| Keywords: |
Human neutrophils; Insect hemocytes; NADPH oxidase; Host defence; Gliotoxin; Phox proteins |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
487 |
| Depositing User: |
Dr. Kevin Kavanagh
|
| Date Deposited: |
11 Apr 2007 |
| Journal or Publication Title: |
Developmental and Comparative Immunology |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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