Nogueira da Costa, Andre and Kenn, Jeffrey N. and Wild, Christopher P. and Findlay, John B. C.
(2011)
An analysis of the phosphoproteome of immune cell lines exposed to the
immunomodulatory mycotoxin deoxynivalenol.
Biochimica et Biophysica Acta - Proteins and Proteomics, 1814 (7).
pp. 850-857.
ISSN 1570-9639
Abstract
The mycotoxin deoxynivalenol (DON) commonly contaminates cereal grains. It is ubiquitous in the Western
European diet, although chronic, low-dose effects in humans are not well described, but immunotoxicity has
been reported. In this study, two-dimensional gel electrophoresis was used to identify phosphoproteomic
changes in human B (RPMI1788) and T (Jurkat E6.1) lymphocyte cell lines after exposure to modest
concentrations of DON (up to 500 ng/mL) for 24 h. Proteins identified as having altered phosphorylation state
post-treatment (C-1-tetrahydrofolate synthase, eukaryotic elongation factor 2, nucleoside diphosphate
kinase A, heat shock cognate 71 kDa protein, eukaryotic translation initiation factor 3 subunit I and growth
factor receptor-bound protein 2) are involved in regulation of metabolic pathways, protein biosynthesis and
signaling transduction. All exhibited a greater than 1.4-fold change, reproducible in three separate
experiments consisting of 36 gels in total. Flow cytometry validated the observations for eukaryotic
elongation factor 2 and growth factor receptor-bound protein 2. These findings provide further insights as to
how low dose exposure to DON may affect human immune function and may have potential as mechanismbased
phosphoprotein biomarkers for DON exposure.
Item Type: |
Article
|
Additional Information: |
The definitive version of this article is available at doi:10.1016/j.bbapap.2011.04.001 |
Keywords: |
Biomarker; Deoxynivalenol; Mycotoxin; Phosphoproteomics; |
Academic Unit: |
Faculty of Science and Engineering > Biology |
Item ID: |
5994 |
Identification Number: |
https://doi.org/10.1016/j.bbapap.2011.04.001 |
Depositing User: |
Professor John Findlay
|
Date Deposited: |
31 Mar 2015 14:47 |
Journal or Publication Title: |
Biochimica et Biophysica Acta - Proteins and Proteomics |
Publisher: |
Elsevier |
Refereed: |
Yes |
URI: |
|
Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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