Helmstaedt, Kerstin and Laubinger, Karen and Vosskuhl, Katja and Bayram, Ozgur and Busch, Silke and Hoppert, Michael and Valerius, Oliver and Seiler, Stephan and Braus, Gerhard H.
(2008)
The Nuclear Migration Protein NUDF/LIS1 Forms a Complex with
NUDC and BNFA at Spindle Pole Bodies.
Eukaryotic Cell, 7 (6).
pp. 1041-1052.
ISSN 1535-9778
Abstract
Nuclear migration depends on microtubules, the dynein motor complex, and regulatory components like
LIS1 and NUDC. We sought to identify new binding partners of the fungal LIS1 homolog NUDF to clarify its
function in dynein regulation. We therefore analyzed the association between NUDF and NUDC in Aspergillus
nidulans. NUDF and NUDC directly interacted in yeast two-hybrid experiments via NUDF’s WD40 domain.
NUDC-green fluorescent protein (NUDC-GFP) was localized to immobile dots in the cytoplasm and at the
hyphal cortex, some of which were spindle pole bodies (SPBs). We showed by bimolecular fluorescence
complementation microscopy that NUDC directly interacted with NUDF at SPBs at different stages of the cell
cycle. Applying tandem affinity purification, we isolated the NUDF-associated protein BNFA (for binding to
NUDF). BNFA was dispensable for growth and for nuclear migration. GFP-BNFA fusions localized to SPBs at
different stages of the cell cycle. This localization depended on NUDF, since the loss of NUDF resulted in the
cytoplasmic accumulation of BNFA. BNFA did not bind to NUDC in a yeast two-hybrid assay. These results
show that the conserved NUDF and NUDC proteins play a concerted role at SPBs at different stages of the cell
cycle and that NUDF recruits additional proteins specifically to the dynein complex at SPBs.
| Item Type: |
Article
|
| Keywords: |
Nuclear Migration; Protein; NUDF/LIS1; Complex; NUDC; BNFA; Spindle Pole Bodies; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6243 |
| Identification Number: |
https://doi.org/10.1128/EC.00071-07 |
| Depositing User: |
Ozgur Bayram
|
| Date Deposited: |
10 Jul 2015 15:54 |
| Journal or Publication Title: |
Eukaryotic Cell |
| Publisher: |
American Society for Microbiology |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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