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    The Nuclear Migration Protein NUDF/LIS1 Forms a Complex with NUDC and BNFA at Spindle Pole Bodies

    Helmstaedt, Kerstin and Laubinger, Karen and Vosskuhl, Katja and Bayram, Ozgur and Busch, Silke and Hoppert, Michael and Valerius, Oliver and Seiler, Stephan and Braus, Gerhard H. (2008) The Nuclear Migration Protein NUDF/LIS1 Forms a Complex with NUDC and BNFA at Spindle Pole Bodies. Eukaryotic Cell, 7 (6). pp. 1041-1052. ISSN 1535-9778

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    Nuclear migration depends on microtubules, the dynein motor complex, and regulatory components like LIS1 and NUDC. We sought to identify new binding partners of the fungal LIS1 homolog NUDF to clarify its function in dynein regulation. We therefore analyzed the association between NUDF and NUDC in Aspergillus nidulans. NUDF and NUDC directly interacted in yeast two-hybrid experiments via NUDF’s WD40 domain. NUDC-green fluorescent protein (NUDC-GFP) was localized to immobile dots in the cytoplasm and at the hyphal cortex, some of which were spindle pole bodies (SPBs). We showed by bimolecular fluorescence complementation microscopy that NUDC directly interacted with NUDF at SPBs at different stages of the cell cycle. Applying tandem affinity purification, we isolated the NUDF-associated protein BNFA (for binding to NUDF). BNFA was dispensable for growth and for nuclear migration. GFP-BNFA fusions localized to SPBs at different stages of the cell cycle. This localization depended on NUDF, since the loss of NUDF resulted in the cytoplasmic accumulation of BNFA. BNFA did not bind to NUDC in a yeast two-hybrid assay. These results show that the conserved NUDF and NUDC proteins play a concerted role at SPBs at different stages of the cell cycle and that NUDF recruits additional proteins specifically to the dynein complex at SPBs.

    Item Type: Article
    Keywords: Nuclear Migration; Protein; NUDF/LIS1; Complex; NUDC; BNFA; Spindle Pole Bodies;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6243
    Identification Number:
    Depositing User: Ozgur Bayram
    Date Deposited: 10 Jul 2015 15:54
    Journal or Publication Title: Eukaryotic Cell
    Publisher: American Society for Microbiology
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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