MURAL - Maynooth University Research Archive Library

    Deletion and Allelic Exchange of the Aspergillus fumigatus veA Locus via a Novel Recyclable Marker Module

    Krappman, Sven and Bayram, Ozgur and Braus, Gerhard H. (2005) Deletion and Allelic Exchange of the Aspergillus fumigatus veA Locus via a Novel Recyclable Marker Module. Eukaryotic Cell, 4 (7). pp. 1298-1307. ISSN 1535-9778

    Download (400kB) | Preview

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular biology. For the generation of targeted gene deletions in the opportunistic pathogen Aspergillus fumigatus we designed a novel blaster module allowing dominant selection of transformants due to resistance to phleomycin as well as dominant (counter)selection of a Cre recombinase-mediated marker excision event. For validation purposes we have deleted the A. fumigatus pabaA gene in a wild-type isolate by making use of this cassette. The resulting pabaA::loxP strain served as the recipient for subsequent targeting of the velvet locus. Homologous reconstitution of the deleted gene was performed by an allele whose expression is driven in a nitrogen source-dependent manner, as validated by Northern analyses. Overexpression of the veA locus in A. fumigatus does not result in any obvious phenotype, whereas the sporulation capacities of the veA null mutant are reduced on nitratecontaining medium, a phenotype that is completely restored in the reconstituted strain.

    Item Type: Article
    Keywords: Deletion; Allelic Exchange; Aspergillus fumigatus; veA Locus; Novel Recyclable Marker Module;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6249
    Identification Number:–1307.2005
    Depositing User: Ozgur Bayram
    Date Deposited: 14 Jul 2015 15:32
    Journal or Publication Title: Eukaryotic Cell
    Publisher: American Society for Microbiology
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads