Weber, Tom S. and Perie, Leila and Duffy, Ken R. (2015) Inferring average generation via division-linked labeling. Working Paper. arXiv.

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Abstract

For proliferating cells subject to both division and death, how can one estimate the average generation number of the living population without continuous observation or a division-diluting dye? In this paper we provide a method for cell systems such that at each division there is an unlikely, heritable one-way label change that has no impact other than to serve as a distinguishing marker. If the probability of label change per cell generation can be determined and the proportion of labeled cells at a given time point can be measured, we establish that the average generation number of living cells can be estimated. Crucially, the estimator does not depend on knowledge of the statistics of cell cycle, death rates or total cell numbers. We validate the estimator and illustrate its features through comparison with published data and physiologically parameterized stochastic simulations, using it to suggest new experimental designs.

Item Type:	Monograph (Working Paper)
Keywords:	average generation; division-linked labeling; cell systems;
Academic Unit:	Faculty of Science and Engineering > Research Institutes > Hamilton Institute
Item ID:	6764
Depositing User:	Dr Ken Duffy
Date Deposited:	11 Jan 2016 16:42
Publisher:	arXiv
URI:
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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