Dowling, Paul and Clarke, Colin and Hennessy, Kim and Torralbo-Lopez, Beatriz and Ballot, Jo and Crown, John and Kiernan, Ingrid and O'Byrne, Kenneth J. and Kennedy, M. John and Lynch, Vincent and Clynes, Martin
(2012)
Analysis of acute-phase proteins, AHSG, C3, CLI, HP and SAA,
reveals distinctive expression patterns associated with breast,
colorectal and lung cancer.
International Journal of Cancer, 131 (4).
pp. 911-923.
ISSN 0020-7136
Abstract
Early detection, clinical management and disease recurrence monitoring are critical areas in cancer treatment in which specific
biomarker panels are likely to be very important in each of these key areas. We have previously demonstrated that levels of
alpha-2-heremans-schmid-glycoprotein (AHSG), complement component C3 (C3), clusterin (CLI), haptoglobin (HP) and serum
amyloid A (SAA) are significantly altered in serum from patients with squamous cell carcinoma of the lung. Here, we report
the abundance levels for these proteins in serum samples from patients with advanced breast cancer, colorectal cancer (CRC)
and lung cancer compared to healthy controls (age and gender matched) using commercially available enzyme-linked
immunosorbent assay kits. Logistic regression (LR) models were fitted to the resulting data, and the classification ability of
the proteins was evaluated using receiver-operating characteristic curve and leave-one-out cross-validation (LOOCV). The most
accurate individual candidate biomarkers were C3 for breast cancer [area under the curve (AUC) 5 0.89, LOOCV 5 73%], CLI
for CRC (AUC 5 0.98, LOOCV 5 90%), HP for small cell lung carcinoma (AUC 5 0.97, LOOCV 5 88%), C3 for lung
adenocarcinoma (AUC 5 0.94, LOOCV 5 89%) and HP for squamous cell carcinoma of the lung (AUC 5 0.94, LOOCV 5 87%).
The best dual combination of biomarkers using LR analysis were found to be AHSG 1 C3 (AUC 5 0.91, LOOCV 5 83%) for
breast cancer, CLI 1 HP (AUC 5 0.98, LOOCV 5 92%) for CRC, C3 1 SAA (AUC 5 0.97, LOOCV 5 91%) for small cell lung
carcinoma and HP 1 SAA for both adenocarcinoma (AUC 5 0.98, LOOCV 5 96%) and squamous cell carcinoma of the lung
(AUC 5 0.98, LOOCV 5 84%). The high AUC values reported here indicated that these candidate biomarkers
have the potential to discriminate accurately between control and cancer groups both individually and in combination with
other proteins.
| Item Type: |
Article
|
| Additional Information: |
The definitive published version of this article is available at DOI: 10.1002/ijc.26462 |
| Keywords: |
acute-phase proteins; biomarkers; cancer; proteomics; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6868 |
| Identification Number: |
https://doi.org/10.1002/ijc.26462 |
| Depositing User: |
Paul Dowling
|
| Date Deposited: |
20 Jan 2016 10:35 |
| Journal or Publication Title: |
International Journal of Cancer |
| Publisher: |
Wiley |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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