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    Demodex-associated bacterial proteins induce neutrophil activation

    O'Reilly, N. and Bergin, David and Reeves, E.P. and McElvaney, N.G. and Kavanagh, Kevin (2012) Demodex-associated bacterial proteins induce neutrophil activation. British Journal of Dermatology, 166 (4). pp. 753-760. ISSN 0007-0963

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    Background: Patients with rosacea demonstrate a higher density of Demodex mites in their skin than do controls. A bacterium isolated from a Demodex mite from a patient with papulopustular rosacea (PPR) was previously shown to provoke an immune response in patients with PPR or ocular rosacea, thus suggesting a possible role for bacterial proteins in the aetiology of this condition. Objectives: To examine the response of neutrophils to proteins derived from a bacterium isolated from a Demodex mite. Methods Bacterial cells were lysed and proteins were partially purified by ÄKTA fast protein liquid chromatography. Isolated neutrophils were exposed to bacterial proteins and monitored for alterations in migration, degranulation and cytokine production. Results: Neutrophils exposed to proteins from Bacillus cells demonstrated increased levels of migration and elevated release of matrix metalloprotease 9, an enzyme known to degrade collagen, and cathelicidin, an antimicrobial peptide. In addition, neutrophils exposed to the bacterial proteins demonstrated elevated rates of interleukin 8 and tumour necrosis factor-a production. Conclusions Proteins produced by a bacterium isolated from a Demodex mite have the ability to increase the migration, degranulation and cytokine production abilities of neutrophils. These results suggest that bacteria may play a role in the inflammatory erythema associated with rosacea.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1111/j.1365-2133.2011.10746.x
    Keywords: Demodex; bacterial proteins; neutrophil activation; rosacea;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6888
    Identification Number:
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 20 Jan 2016 13:21
    Journal or Publication Title: British Journal of Dermatology
    Publisher: Wiley
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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