Smith, Alanna and McCann, Malachy and Kavanagh, Kevin
(2013)
Proteomic analysis of the proteins released from Staphylococcus aureus
following exposure to Ag(I).
Toxicology in Vitro, 27.
pp. 1644-1648.
ISSN 0887-2333
Abstract
The silver ion (Ag(I)) has well established antimicrobial properties and is widely used in a variety of antibacterial
ointments and plasters for the control of wound infections. Wounds are frequently colonised by
the bacterium Staphylococcus aureus and the aim of the work presented here was to establish how S. aureus
responded following exposure to Ag(I). Exposure of S. aureus to Ag(I) resulted in the release of a range
of proteins from cells. Analysis of proteins released revealed a number of proteins associated with the
stress response (e.g. alkaline shock protein, methionine sulfoxide reductase), virulence (e.g. signal transduction
protein) and metabolism (e.g. lipase, acetate kinase, phosphoglycerate mutase). The release of
toxins (e.g. a-hemolysin, bifunctional autolysin, leucocidin F) was decreased. These results indicated that,
while silver is a potent antimicrobial agent, exposure of S. aureus to this metal results in the release of a
variety of proteins from the cell. Many of the proteins showing increased release were antigenic and
would have the potential to induce an inflammatory response at the site of infection and thus delay
healing.
| Item Type: |
Article
|
| Additional Information: |
The definitive published version of this article is available at DOI: 10.1016/j.tiv.2013.04.007 |
| Keywords: |
Anti-microbial; Bacterial colonisation; Oxidative stress; Proteomics; Silver; Staphylococcus; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6892 |
| Identification Number: |
https://doi.org/10.1016/j.tiv.2013.04.007 |
| Depositing User: |
Dr. Kevin Kavanagh
|
| Date Deposited: |
20 Jan 2016 13:20 |
| Journal or Publication Title: |
Toxicology in Vitro |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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