Keady, S.M. and Kenny, D.A. and Ohlendieck, Kay and Doyle, Sean and Keane, M.G. and Waters, S.M.
(2013)
Proteomic profiling of bovine M. longissimus lumborum from Crossbred Aberdeen
Angus and Belgian Blue sired steers varying in genetic merit for carcass weight.
Journal of Animal Science, 91 (2).
pp. 654-665.
ISSN 0021-8812
Abstract
Bovine skeletal muscle is a tissue
of significant value to the beef industry and global
economy. Proteomic analyses offer the opportunity to
detect molecular mechanisms regulating muscle growth
and intramuscular fat accumulation. The current study
aimed to investigate differences in protein abundance
in skeletal muscle tissue of cattle from two breeds of
contrasting maturity (early vs. late maturing), adiposity,
and muscle growth potential, namely, Belgian Blue
(BB) × Holstein Friesian and Aberdeen Angus (AA) ×
Holstein Friesian. Twenty AA (n = 10) and BB (n = 10)
sired steers, the progeny of sires of either high or low
genetic merit, expressed as expected progeny difference
for carcass weight (EPDcwt), and bred through
AI, were evaluated as 4 genetic groups, BB-High,
BB-Low, AA-High, and AA-Low (n = 5 per treatment).
Chemical composition analysis of M. longissimus
lumborum showed greater protein and moisture and
decreased lipid concentrations for BB-sired compared
with AA-sired steers. To investigate the effects
of both sire breed and EPDcwt on M. longissimus
lumborum, proteomic analysis was performed using
2-dimensional difference gel electrophoresis followed by mass spectrometry. Proteins were identified from
their peptide sequences, using the National Center for
Biotechnology Information (NCBI) and Swiss-prot
databases. Metabolic enzymes involved in glycolysis
(glycogen phosphorylase, phosphoglycerate mutase)
and the citric acid cycle (aconitase 2, oxoglutarate
dehydrogenase) were increased in AA- vs. BB-sired
steers. Expression of proteins involved in cell structure,
such as myosin light chain isoforms and troponins I
and T, were also altered due to sire breed. Furthermore,
heat shock protein β-1 and peroxiredoxin 6, involved
in cell defense, had increased abundance in muscle of
AA-sired relative to BB-sired steers. Protein abundance
of glucose-6-phosphate isomerase, enolase-3, and
pyruvate kinase was greater in AA-sired animals of
High compared with Low EPDcwt. Changes in the
expression of these proteins were supported by gene
expression analysis using quantitative real-time PCR.
This information will aid in our understanding of
genetic infl uences controlling muscle growth and fat
accumulation and could contribute to future breeding
programs to increase lean tissue gain of beef cattle.
| Item Type: |
Article
|
| Additional Information: |
The definitive published version of this article is available at Keady, Sarah M.; Kenny, David A.; Ohlendieck, Kay; Doyle, Sean; Keane, M. G.; Waters, Sinéad M., ‘Proteomic profiling of bovine M. longissimus lumborum from Crossbred Aberdeen Angus and Belgian Blue sired steers varying in genetic merit for carcass weight’ Journal of Animal Science, Vol. 91, no. 2, 2013, pp. 654-665. DOI: 10.2527/jas.2012-5850 |
| Keywords: |
cattle; growth; proteomics; skeletal muscle; glycolysis; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6911 |
| Identification Number: |
https://doi.org/10.2527/jas.2012-5850 |
| Depositing User: |
Prof. Kay Ohlendieck
|
| Date Deposited: |
21 Jan 2016 14:39 |
| Journal or Publication Title: |
Journal of Animal Science |
| Publisher: |
American Society of Animal Science |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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