O'Connell, Kathleen and Prencipe, Maria and O'Neill, Amanda and Corcoran, Claire and Rani, Sweta and Henry, Michael and Dowling, Paul and Meleady, Paula and O'Driscoll, Lorraine and Watson, William and O'Connor, Robert
(2012)
The use of LC-MS to identify differentially expressed
proteins in docetaxel-resistant prostate cancer cell lines.
Proteomics, 12 (13).
pp. 2115-2126.
ISSN 1615-9853
Abstract
Docetaxel is a taxane-derived chemotherapy drug that has been approved for treatment of
prostate cancer. While docetaxel is frequently used as a treatment for hormone-refractory
prostate cancer, a subset of patients either do not respond to this treatment or those that do
respond eventually become resistant to the drug over time. Resistance to docetaxel is complex
and multi-factoral and further understanding of the cellular biochemistry underlying resistance
is vital to improve treatment efficacy. To identify proteins altered in the resistant phenotype,
three parental cell lines DU145, 22RV1 and PC-3, as well as their docetaxel resistant sub-lines,
were subjected to quantitative label-free LC-MS proteomic profiling. A total of 189 significant
(p < 0.05) protein abundance changes were identified in the DU145 resistant sub-lines, 254 in
the 22RV1 sub-lines, and 51 and 72 in the 8 and 12 nM resistant PC-3 sub-lines, respectively.
From these, 29 proteins demonstrated a significant (p < 0.05) fold change across two or more
resistant variants. These included proteins indicative of an epithelial-to-mesenchemyl transition
as well as altered heat shock response elements.
| Item Type: |
Article
|
| Additional Information: |
The definitive published version of this article is available at DOI: 10.1002/pmic.201100489 |
| Keywords: |
Cancer; Cell biology; Docetaxel; Prostate; Resistant; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6926 |
| Identification Number: |
https://doi.org/10.1002/pmic.201100489 |
| Depositing User: |
Paul Dowling
|
| Date Deposited: |
26 Jan 2016 17:32 |
| Journal or Publication Title: |
Proteomics |
| Publisher: |
Wiley |
| Refereed: |
Yes |
| Funders: |
Science Foundation Ireland (SFI) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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