Keenan, Joanne and Joyce, Helena and Aherne, Sinead and O'Dea, Shirley and Doolan, Padraig and Lynch, Vincent and Clynes, Martin (2012) Olfactomedin III expression contributes to anoikis-resistance in clonal variants of a human lung squamous carcinoma cell line. Experimental Cell Research, 318 (5). pp. 593-602. ISSN 0014-4827

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Abstract

Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respectively). DLKP-I shows increased apoptosis consistently over all time points tested while DLKP-M appear to slow down metabolically and perhaps delays onset of anoikis by undergoing autophagy. Expression microarray analysis identified pronounced differential expression of Olfactomedin 3 (OLFM3) between the clones. High expression of OLFM3 was confirmed at the RNA level by qRT-PCR in DLKP-SQ and at the protein level by Western blotting (within the cell and secreted). Little or no OLFM3 was detected in the other two clones (DLKPM and DLKP-I). Following siRNA knockdown of OLFM3 in DLKP-SQ, anoikis was increased 2.8- fold to 21% which was intermediate between the anoikis levels in DLKP-SQ and DLKP-M or DLKP-I. This knockdown correlated with increased apoptosis in suspension but not in attached culture conditions. Addition of recombinant OLFM3 reduced anoikis in DLKP-I. This is the first instance of OLFM3 being linked with anoikis resistance in a human cancer cell line.

Item Type:	Article
Additional Information:	The definitive published version of this article is available at DOI: 10.1016/j.yexcr.2012.01.012
Keywords:	Olfactomedin 3; Anoikis; Lung cancer;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Institute of Immunology
Item ID:	6930
Identification Number:	https://doi.org/10.1016/j.yexcr.2012.01.012
Depositing User:	Dr. Shirley O'Dea
Date Deposited:	29 Jan 2016 15:27
Journal or Publication Title:	Experimental Cell Research
Publisher:	Elsevier
Refereed:	Yes
Funders:	Higher Education Authority (HEA), Science Foundation Ireland (SFI)
URI:	http://www.sciencedirect.com/
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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