Kavanagh, H, and Noone, C. and Cahill, Emer and English, Karen and Locht, C. and Mahon, Bernard P. (2010) Attenuated Bordetella pertussis vaccine strain BPZE1 modulates allergen-induced immunity and prevents allergic pulmonary pathology in a murine model. Clinical & Experimental Allergy, 40 (6). pp. 933-941. ISSN 1365-2222

Preview

Download (1MB) | Preview

Abstract

Background Virulent Bordetella pertussis, the causative agent of whooping cough, exacerbates allergic airway inflammation in a murine model of ovalbumin (OVA) sensitization. A live genetically attenuated B. pertussis mucosal vaccine, BPZE1, has been developed that evokes full protection against virulent challenge in mice but the effect of this attenuated strain on the development of allergic responses is unknown. Objective To assess the influence of attenuated B. pertussis BPZE1 on OVA priming in a murine model of allergic airway inflammation. Methods Mice were challenged with virulent or attenuated strains of B. pertussis, and sensitized to allergen (OVA) at the peak of bacterial carriage. Subsequently, airway pathology, local inflammation and OVA-specific immunity were examined. Results In contrast to virulent B. pertussis, live BPZE1 did not exacerbate but reduced the airway pathology associated with allergen sensitization. BPZE1 immunization before allergen sensitization did not have an adjuvant effect on allergen specific IgE but resulted in a statistically significant decrease in airway inflammation in tissue and bronchoalveolar lavage fluid. BPZE1 significantly reduced the levels of OVA-driven IL-4, IL-5 and IL-13 but induced a significant increase in IFN-g in response to OVA re-stimulation. Conclusions These data demonstrate that, unlike virulent strains, the candidate attenuated B. pertussis vaccine BPZE1 does not exacerbate allergen-driven airway pathology. BPZE1 may represent an attractive T-helper type 1 promoting vaccine candidate for eradication of whooping cough that is unlikely to promote atopic disease.

Item Type:	Article
Additional Information:	This work is part of the CHILD-INNOVAC network (Collaborative Project) supported by the European Commission under the Health Cooperation Work Programme of the 7th Framework Programme (Grant agreement no. 201502). H. K. is funded by the Irish Health Research Board.
Keywords:	asthma; Bordetella pertussis; vaccine; whooping cough;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7145
Identification Number:	https://doi.org/10.1111/j.1365-2222.2010.03459.x
Depositing User:	Bernard Mahon
Date Deposited:	01 Jul 2016 09:05
Journal or Publication Title:	Clinical & Experimental Allergy
Publisher:	Blackwell Publishing Ltd.
Refereed:	Yes
Funders:	European Commission, Health Research Board (HRB)
URI:	http://onlinelibrary.wiley.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

Repository Staff Only(login required)

Item control page

Downloads

Origin of downloads