Meager, Anthony and Parti, Sharan and Leung, Helen and Peil, Elizabeth and Mahon, Bernard P.
(1987)
Preparation and Characterization of
Monoclonal Antibodies Directed Against
Antigenic Determinants of Recombinant
Human Tumour Necrosis Factor (rTNF).
Hybridoma, 6 (3).
pp. 305-311.
ISSN 1554-0014
Abstract
A large number of monoclonal antibodies (McAb) binding to
antigenic determinants of human tumour necrosis factor (TNF) were
prepared from two fusions of mouse myeloma NSO cells with spleen
cells from Balb/c mice immunized with highly purified recombinant
(r)TNF. Several of these McAbs were highly neutralizing with
respect to the biological activity (cytotoxicity) of TNF manifested
in L-929 C1.10 cells. Antibody competition experiments suggested
the presence of at least two antigenic determinants on the rTNF
molecule through which binding of McAb effects neutralization of
biological activity. Some of these McAbs were shown to be suitable
for the development of immuoassays to quantify rTNF.
| Item Type: |
Article
|
| Keywords: |
Preparation; Characterization;
Monoclonal Antibodies; Antigenic Determinants; Recombinant Human Tumour Necrosis Factor; rTNF; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7160 |
| Depositing User: |
Bernard Mahon
|
| Date Deposited: |
06 Jul 2016 09:59 |
| Journal or Publication Title: |
Hybridoma |
| Publisher: |
Mary Ann Lieber!, Inc. |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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