Türkela, Sezai and Bayram, Ozgur and Arika, Elif
(2009)
Glucose Signaling Pathway and Growth Conditions Regulate Gene
Expression in Retrotransposon Ty2.
Zeitschrift für Naturforschung C: a Journal of Biosciences, 64 (7-8).
pp. 526-532.
ISSN 0939-5075
Abstract
Gene expression in the yeast retrotransposon Ty2 is regulated at transcriptional and translational
levels. In this study, we have shown that the transcription of Ty2 is partially dependent
on the membrane-bound glucose sensors Gpr1p and Mth1p in Saccharomyces cerevisiae.
Transcription of Ty2 decreased approx. 3-fold in the gpr1, mth1 yeast mutant. Moreover,
our results revealed that the transcription of Ty2 fl uctuates during the growth stages of
S. cerevisae. Both transcription and the frameshift rate of Ty2 rapidly dropped when the
stationary stage yeast cells were inoculated into fresh medium. There was an instant activation
of Ty2 transcription and a high level expression during the entire logarithmic stage of
yeast growth. However, the transcription of Ty2 decreased 2-fold when the yeast cultures
entered the stationary stage. The frameshift rate in Ty2 also varied depending on the growth
conditions. The highest frameshift level was observed during the mid-logarithmic stage. It
decreased up to 2-fold during the stationary stage. Furthermore, we have found that the
frameshift rate of Ty2 diminished at least 5-fold in slowly growing yeasts. These results indicate
that the transcription and the frameshift effi ciency are coordinately regulated in the
retrotransposon Ty2 depending on the growth conditions of S. cerevisiae.
| Item Type: |
Article
|
| Keywords: |
Frameshift; Ty Elements; Glucose Sensing; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7181 |
| Depositing User: |
Ozgur Bayram
|
| Date Deposited: |
13 Jul 2016 12:02 |
| Journal or Publication Title: |
Zeitschrift für Naturforschung C: a Journal of Biosciences |
| Publisher: |
de Gruyter |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
Repository Staff Only(login required)
 |
Item control page |
Downloads per month over past year
Origin of downloads
Altmetric
Altmetric