Dowling, Paul and O'Driscoll, Lorraine and O'Sullivan, Finbarr and Dowd, Andrew and Henry, Michael and Jeppesen, Per Bendix and Meleady, Paula and Clynes, Martin
(2006)
Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress.
Proteomics, 6 (24).
pp. 6578-6587.
ISSN 1615-9853
Abstract
The glucose-sensitive insulin-secretion (GSIS) phenotype is relatively unstable in long-term culture
of beta cells. The purpose of this study was to investigate relative changes in the proteome
between glucose-responsive (low passage) and glucose non-responsive (high passage) murine
MIN-6 pancreatic beta cells. The 2D-DIGE and subsequent DeCyder analysis detected 3351 protein
spots in the pH range of 4–7. Comparing MIN-6(H) to MIN-6(L) and using a threshold of
1.2-fold, the number of proteins with a decrease in expression level was 152 (4.5%), similar was
3140 (93.7%) and increased 59 (1.8%). From the differentially expressed proteins identified in
this study, groups of proteins associated with the endoplasmic reticulum (ER) and proteins
involved in oxidative stress were found to be significantly decreased in the high-passage (H passage)
cells. These proteins included endoplasmic reticulum protein 29 (ERp29); 78-kDa glucoserelated
protein, (GRP78); 94-kDa glucose-related protein (GRP94); protein disulphide isomerase;
carbonyl reductase 3; peroxidoxin 4 and superoxide dismutase 1. These results suggest that non-
GSIS MIN-6 cells do not have the same ability/capacity of glucose-responsive MIN-6 cells to
successfully fold, modify or secrete proteins and counteract the problems associated with oxidative
stress.
| Item Type: |
Article
|
| Keywords: |
DIGE; Endoplasmic reticulum; Glucose-Sensitive Insulin Secretion; MIN-6 beta cells; Oxidative stress; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7350 |
| Identification Number: |
https://doi.org/10.1002/pmic.200600298 |
| Depositing User: |
Paul Dowling
|
| Date Deposited: |
16 Aug 2016 15:43 |
| Journal or Publication Title: |
Proteomics |
| Publisher: |
Wiley |
| Refereed: |
Yes |
| Funders: |
Higher Education Authority (HEA) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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