Dowling, Paul and Maurya, Priyanka and Meleady, Paula and Glynn, Sharon and Dowd, Andrew and Henry, Michael and Clynes, Martin
(2007)
Purification and Identification of a 7.6-kDa Protein in
Media Conditioned by Superinvasive Cancer Cells.
Anticancer Research, 27 (3A).
pp. 1309-1317.
ISSN 0250-7005
Abstract
Background: Selection of the human drug sensitive
and invasive cell line (MDA-MB-435S-F) with the
chemotherapeutic agent paclitaxel, resulted in the development
of drug resistant cell lines displaying enhanced invasion-related
characteristics. Materials and Methods: Serum-free conditioned
media from the human cancer drug-sensitive and invasive cell
line (MDA-MB-435S-F) and its paclitaxel-resistant superinvasive
variant (MDA-MB-435S-F/Taxol10p4pSI) were analyzed using
Surface enhanced laser desorption/ionization time-of-flight mass
spectrometry (SELDI-TOF MS). Results: A differentially
expressed protein was observed at 7.6 kDa, which was 4-fold upregulated
in MDA-MB-435S-F/Taxol10p4pSI. The differentially
expressed protein was identified using matrix-assisted laser
desorption ionization tandem time-of-flight mass spectrometry
(MALDI-TOF/TOF MS), as a fragment of bovine transferrin.
The transferrin receptor was also found to be overexpressed in the
superinvasive cell line. Conclusion: Cleavage of serum proteins
such as transferrin could provide a valuable source of markers
for malignant tumours and could also play a role in aspects of
cancer pathogenesis, such as tumour cachexia.
| Item Type: |
Article
|
| Keywords: |
Drug resistant; IMAC; SELDI; superinvasive; transferrin; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7355 |
| Depositing User: |
Paul Dowling
|
| Date Deposited: |
18 Aug 2016 15:23 |
| Journal or Publication Title: |
Anticancer Research |
| Publisher: |
The International Institute of Anticancer Research |
| Refereed: |
Yes |
| Funders: |
Higher Education Authority (HEA), Science Foundation Ireland (SFI) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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