Dowling, Paul and O'Driscoll, Lorraine and Meleady, Paula and Henry, Michael and Roy, Shunil and Ballot, Jo and Moriarty, Michael and Crown, John and Clynes, Martin (2007) 2-D difference gel electrophoresis of the lung squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of ten specific proteins. Electrophoresis, 28 (23). pp. 4302-4310. ISSN 0173-0835

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Abstract

Most lung cancers are diagnosed too late for curative treatment to be possible, therefore early detection is crucial. Serum proteins are a rich source of biomarkers and have the potential to be used as diagnostic and prognostic indicators for lung cancer. In order to examine differences in serum levels of specific proteins associated with human lung squamous carcinoma, immunodepletion of albumin and five other high-abundant serum proteins followed by 2-D difference gel electrophoresis (DIGE) analysis and subsequent MS was used to generate a panel of proteins found to be differentially expressed between the cancer and normal samples. Proteins found to have increased abundance levels in squamous cell carcinoma sera compared to normal sera included apolipoprotein A-IV precursor, chain F; human complement component C3c, haptoglobin, serum amyloid A protein precursor and Ras-related protein Rab-7b. Proteins found to have lower abundance levels in squamous cell carcinoma sera compared to normal sera included alpha-2-HS glycoprotein, hemopexin precursor, proapolipoprotein, antithrombin III and SP40; 40. The data presented here demonstrate that high-abundant protein removal combined with 2-D DIGE is a powerful strategy for the discovery of potential biomarkers. The identification of lung cancer-specific biomarkers is crucial to early detection, which in turn could lead to a dramatic increase in survival rates.

Item Type:	Article
Keywords:	Biomarker; Difference gel electrophoresis; Immunodepletion; Serum; Squamous;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7359
Identification Number:	https://doi.org/10.1002/elps.200700246
Depositing User:	Paul Dowling
Date Deposited:	18 Aug 2016 15:21
Journal or Publication Title:	Electrophoresis
Publisher:	Wiley-VCH Verlag
Refereed:	Yes
Funders:	Higher Education Authority (HEA)
URI:	http://onlinelibrary.wiley.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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