Jansen, Marcel A.K. and Elfstrand, Malin and Heggie, Laura and Sitbon, Folke and Dix, Philip and Thorneley, Roger N.F. (2004) Over-expression of phenol-oxidising peroxidases alters the UV-susceptibility of transgenic Nicotiana tabacum. New Phytologist, 163 (3). pp. 585-594. ISSN 0028-646X

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Abstract

* Class III peroxidases catalyse the oxidative crosslinking of UV-absorbing phenolics. The effect of changes in the activity of phenol oxidising peroxidases (EC 1.11.1.7) on UV-tolerance in Nicotiana tabacum plants has been determined. * The UV-sensitivity of transgenic N. tabacum lines, altered in their peroxidase expression pattern, was studied by measuring radiation effects on photosynthetic efficiency. * Analysis of the effect of UV-radiation on the relative variable chlorophyll fluorescence showed that the SPI-2 line, which over-expresses a defence-related cationic peroxidase, is markedly UV-tolerant. By contrast, the ROPN3-line, which overexpresses a synthetic horseradish peroxidase-C gene, was found to be UV-sensitive. The increased activity of indole-3-acetic acid (IAA) inducible peroxidases in homozygous IAA-overproducing transgenic plants was also found to correlate with UV-sensitivity. * It is concluded that only specific peroxidase isozymes, through their effects on phenolic metabolism, contribute to the UV protection response. Thus, the analysis of the role of isozymes in UV-protection addresses fundamental questions of isozyme diversity and/or redundancy in relation to phenolic substrates

Item Type:	Article
Keywords:	UV-radiation; auxin; stress; peroxidase; chlorophyll afluorescence; Nicotiana tabacum; tobacco;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7372
Identification Number:	https://doi.org/10.1111/j.1469-8137.2004.01132.x
Depositing User:	Prof. Philip J. Dix
Date Deposited:	24 Aug 2016 15:38
Journal or Publication Title:	New Phytologist
Publisher:	Wiley
Refereed:	Yes
URI:	http://onlinelibrary.wiley.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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