Parkinson, M. and Cotter, T. and Dix, Philip
(1990)
Peroxidase production by cell suspension and hairy root cultures of horseradish (Armoracia rusticana).
Plant Science, 66 (2).
pp. 271-277.
ISSN 0168-9452
Abstract
The potential of cell suspension and ‘hairy root’ cultures of horseradish for production of peroxidase was assessed and the factors limiting production in culture were examined. Peroxidase activity is closely related to growth in both cell suspension cultures and ‘hairy root’ cultures of horseradish. Under the growth conditions used in the present study however, cell suspension cultures produce twice the total activity of ‘hairy root’ cultures, and up to 8% of this activity is released into the culture filtrate at high specific activity. The growth and peroxidase activity of cell suspension cultures are limited by both phosphate and sucrose and the rate of oxygenation of the culture is critical for maximising growth rate, biomass accumulation and peroxidase activity. The isoelectric focussing banding pattern of peroxidase from the ‘hairy root’ cultures was identical with commercially available horseradish peroxidase whilst that from cell suspension cultures differed with an additional two bands at high pI.
| Item Type: |
Article
|
| Keywords: |
peroxidase; cell suspension; ‘hairy root’; horseradish; Armoracia rusticana; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7374 |
| Identification Number: |
https://doi.org/10.1016/0168-9452(90)90213-8 |
| Depositing User: |
Prof. Philip J. Dix
|
| Date Deposited: |
24 Aug 2016 15:38 |
| Journal or Publication Title: |
Plant Science |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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