Pellegrineschi, A. and Kist, M. and Dix, Ilona and Kavanagh, T.A. and Dix, Philip
(1995)
Expression of horseradish peroxidase in transgenic tobacco.
Biochemical Society Transactions, 23 (2).
pp. 247-250.
ISSN 0300-5127
Abstract
Peroxidases are common oxidoreductases present
in plants, animals and micro-organisms. Plant peroxidases
are classified as basic, neutral or acidic
enzymes, depending on their profiles of elution
during ion-exchange column chromatography or
isoelectric points [ 11. Horseradish (Amtoru~hru sticuna)
peroxidases (HRPs; EC 1.1 1.1.7), are
probably the best characterized of these enzymes.
They are believed to be involved in plant responses
to a number of biotic and abiotic stresses, notably in
the disruption of free radicals and active derivatives
of oxygen. The effects of these molecules have been
well described [2], and in general the presence of
these compounds are ‘highly destructive’ for cell
metabolism. However, these oxygen derivatives are
involved in halogenation and hydroxylation reactions
in peroxisomes and in the cell wall, suggesting
that the plant cell needs a fine regulation between
production and destruction of oxygen radicals. A
key to the comprehension of the role of peroxidase
activity in this and other stress responses could be
the study of the effect of modified expression and
targeting of peroxidase activity in transgenic plants.
The present report describes the production of
such tobacco plants using a synthetic HRP gene [ 31,
with a range of regulatory and targeting sequences.
| Item Type: |
Article
|
| Keywords: |
Expression; horseradish peroxidase; transgenic tobacco; oxidoreductases; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7387 |
| Identification Number: |
https://doi.org/10.1042/bst0230247 |
| Depositing User: |
Prof. Philip J. Dix
|
| Date Deposited: |
26 Aug 2016 10:43 |
| Journal or Publication Title: |
Biochemical Society Transactions |
| Publisher: |
Portland Press |
| Refereed: |
No |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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