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    Correlation between gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella

    Reeves, Emer P. and Messina, C.G.M. and Doyle, Sean and Kavanagh, Kevin (2004) Correlation between gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella. Mycopathologia, 158 (1). pp. 73-79. ISSN 0301-486X

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    Aspergillus fumigatus is a pathogenic fungus capable of causing both allergic lung disease and invasive aspergillosis, a serious, life-threatening condition in neutropenic patients. Aspergilli express an array of mycotoxins and enzymes which may facilitate fungal colonisation of host tissue. In this study we investigated the possibility of using the insect, Galleria mellonella, for in vivo pathogenicity testing of Aspergillus species. Four clinical isolates of Aspergillus fumigatus and a single strain of Aspergillus niger were characterised for catalase and elastase activity and for the production of gliotoxin. Gliotoxin is an immunosuppressive agent previously implicated in assisting tissue penetration. Results illustrated a strain dependent difference in elastase activity but no significant difference in catalase activity. Gliotoxin production was detected in vitro and in vivo by Reversed Phase-High Performance Liquid Chromatography,with highest amounts being produced by A. fumigatus ATCC 26933 (350 ng/mg hyphae). Survival probability plots (Kaplan–Meier) of experimental groups infected with Aspergillus conidia indicate that G. mellonella is more susceptible to fungal infection by A. fumigatus ATCC 26933, implicating a critical role for gliotoxin production rather than growth rate or enzymatic activity in the virulence of A. fumigatus in this model.

    Item Type: Article
    Keywords: gliotoxin; Aspergillus fumigatus; Galleria mellonella; fungal infection; immunosuppression;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7399
    Identification Number:
    Depositing User: Dr. Sean Doyle
    Date Deposited: 29 Aug 2016 15:31
    Journal or Publication Title: Mycopathologia
    Publisher: Springer Verlag
    Refereed: Yes
    Funders: Higher Education Authority (HEA)
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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