Gaffney, Mark and Doyle, Sean and Murphy, Richard (2009) Optimization of Xylanase Production by Thermomyces Ianuginosus in Solid State Fermentation. Bioscience, Biotechnology, and Biochemistry, 74 (12). pp. 2640-2644. ISSN 0916-8451

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Abstract

Extracellular xylanase production by the thermophilic fungus Thermomyces lanuginosus 195 in solid state fermentation (SSF) was found to be significantly affected by fermentation temperature, duration, and inoculum volume (p < or = 0.001). Optimization of these parameters corresponded to a 21.7% increase in xylanase yield. Maximum activity (2,335 U/g of wheat bran) was obtained when 10 g of wheat bran was inoculated with 10 ml of liquid culture and cultivated at 45 degrees C for 40 h. The influence of supplemental carbon and nitrogen sources (3% w/v) on xylanase production was also assessed. Wheat bran, supplemented with glucose and cellulose, facilitated 10% and 7% increases in relative activity respectively. Ammonium based salts, nitrates, and a number of organic nitrogen sources served only to reduce xylanase production (p < or = 0.005) significantly. The enhanced xylanase titers achieved in the present study emphasize the need for optimizing growth conditions for maximum enzyme production in SSF.

Item Type:	Article
Keywords:	xylanase; Thermomyces lanuginosus; optimization; solid state fermentation; SSF; medium supplementation;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7409
Identification Number:	https://doi.org/10.1271/bbb.90493
Depositing User:	Dr. Sean Doyle
Date Deposited:	29 Aug 2016 15:27
Journal or Publication Title:	Bioscience, Biotechnology, and Biochemistry
Publisher:	Taylor & Francis
Refereed:	Yes
URI:	http://www.tandfonline.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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