McBride, Shirley and Rannie, D. and Harrison, D.J. (2000) Gene transfer to adult human lung tissue ex vivo. Gene Therapy, 7 (8). pp. 675-678. ISSN 0969-7128

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Abstract

The potential of gene therapy for treatment of lung disease remains unrealised. Early model systems often resulted in promising efficiency of gene transfer, only to prove irreproducible in the clinic. While problems such as induction of host immune responses and duration of expression also need to be addressed, it is now widely believed that alternative, relevant models which more accurately reflect gene transfer efficiencies in human lungs are urgently required. We report here on a human lung slice culture system to assess gene transfer to adult lung epithelium. A lacZ-expressing adenovirus (AdCA35lacZ) was used as a reporter vector. A solution of AdCA35lacZ was instilled via bronchioles into resected lung tissue, a route analogous to clinical administration. Following a 1 h incubation, the tissue was inflated with a 0.4% agarose solution, instilled via the same bronchioles. Once solidified, 500 mm slices of the tissue were prepared and cultured for 4 days. b-Galactosidase staining revealed lacZ transgene expression in bronchiolar and alveolar cells of the lung slices throughout the 4 days in culture. This system, which can also be used to study other viral and liposome vectors, could prove to be a useful alternative model for assessing gene delivery to adult human lung epithelium.

Item Type:	Article
Keywords:	gene therapy; adenovirus; human; lung;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7415
Depositing User:	Dr. Shirley O'Dea
Date Deposited:	31 Aug 2016 13:57
Journal or Publication Title:	Gene Therapy
Publisher:	Nature Publishing Group
Refereed:	Yes
URI:
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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