Lelivelt, Cilia L.C. and McCabe, Matthew S. and Newell, Christine A. and de Snoo, C. Bastiaan and van Dun, Kees M.P. and Birch-Machin, Ian and Gray, John C. and Mills, Kingston H.G. and Nugent, Jacqueline M. (2005) Stable plastid transformation in lettuce (Lactuca sativa L.). Plant Molecular Biology, 58 (6). pp. 763-774. ISSN 0167-4412

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Abstract

Although plastid transformation in higher plants was first demonstrated in the early 1990s it is only recently that the technology is being extended to a broader range of species. To date, the production of fertile transplastomic plants has been reported for tobacco, tomato, petunia, soybean, cotton and Lesquerella fendleri (Brassicaceae). In this study we demonstrate a polyethylene glycol-mediated plastid transformation system for lettuce that generates fertile, homoplasmic, plastid-transformed lines. Transformation was achieved using a vector that targets genes to the trnA/trnI intergenic region of the lettuce plastid genome employing the aadA gene as a selectable marker against spectinomycin. Spectinomycin resistance and heterologous gene transcription were shown in T(1) plants derived from self-pollinated primary regenerants demonstrating transmission of the plastid-encoded transgene to the first seed generation. Crossing with male sterile wild-type lettuce showed that spectinomycin resistance was not transmitted via pollen. Constructs containing the gfp gene showed plastid-based expression of green fluorescent protein. The lettuce plastid could have potential both as a production and a delivery system for edible human therapeutic proteins.

Item Type:	Article
Keywords:	aadA; lettuce; PEG; plastid transformationt; rnI/trnA;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7421
Identification Number:	https://doi.org/10.1007/s11103-005-7704-8
Depositing User:	Dr. Jacqueline Nugent
Date Deposited:	31 Aug 2016 15:11
Journal or Publication Title:	Plant Molecular Biology
Publisher:	Springer Verlag
Refereed:	Yes
URI:	http://link.springer.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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