Regeai, S.O. and Dolan, K.M. and Fitzpatrick, David A. and Browne, J.A. and Jones, J.T. and Burnell, Ann (2009) Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species. Molecular Ecology Resources, 9 (1). pp. 421-424. ISSN 1755-098X

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Abstract

We describe 24 novel primers that amplify intron regions in housekeeping and structural genes of Heterorhabditis bacteriophora. The cross-amplification potential of these primers in seven other Heterorhabditis species was determined. The results obtained showed interspecific nucleotide, length and splice site variability in the sequenced introns and for one gene, an intron gain was observed. These primers will be useful tools for studying population genetics, genetic diversity and intron DNA evolution within the genus Heterorhabditis and other genera of rhabditid nematodes.

Item Type:	Article
Keywords:	entomopathogenic; EPIC-PCR; Heterorhabditis; intron; molecular markers; nematode;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7446
Identification Number:	https://doi.org/10.1111/j.1755-0998.2008.02431.x
Depositing User:	David Fitzpatrick
Date Deposited:	15 Sep 2016 09:56
Journal or Publication Title:	Molecular Ecology Resources
Publisher:	Blackwell Publishing Ltd.
Refereed:	Yes
URI:	http://onlinelibrary.wiley.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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