Walsh, Fiona and Ingenfeld, A. and Zampicolli, M. and Hilber-Bodmer, M. and Frey, Jurg E. and Duffy, B.
(2011)
Real-time PCR methods for quantitative monitoring of streptomycin and tetracycline
resistance genes in agricultural ecosystems.
Journal of Microbiological Methods, 86 (2).
pp. 150-155.
ISSN 0167-7012
Abstract
Antibiotic application in plant agriculture is primarily used to control fire blight caused by Erwinia amylovora
in pome fruit orchards. In order to facilitate environmental impact assessment for antibiotic applications, we
developed and validated culture-independent quantitative real-time PCR multiplex assays for streptomycin
(strA, strB, aadA and insertion sequence IS1133) and tetracycline (tetB, tetM and tetW) resistance elements in
plant and soil samples. The qPCR were reproducible and consistent whether the DNA was extracted directly
from bacteria, plant and soil samples inoculated with bacteria or soil samples prior to and after manure slurry
treatment. The genes most frequently identified in soils pre- and post-slurry treatment were strB, aadA, tetB
and tetM. All genes tested were detected in soils pre-slurry treatment, and a decrease in relative
concentrations of tetB and the streptomycin resistance genes was observed in samples taken post-slurry
treatment. These multiplex qPCR assays offer a cost-effective, reliable method for simultaneous quantification
of antibiotic resistance genes in complex, environmental sample matrices.
| Item Type: |
Article
|
| Keywords: |
Antibiotic resistance; Streptomycin; Tetracycline; Agriculture; qPCR; TaqMan®; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7475 |
| Identification Number: |
https://doi.org/10.1016/j.mimet.2011.04.011 |
| Depositing User: |
Fiona Walsh
|
| Date Deposited: |
28 Sep 2016 15:30 |
| Journal or Publication Title: |
Journal of Microbiological Methods |
| Publisher: |
Elsevier B.V. |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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