Roche, C. and Boo, T.W. and Walsh, Fiona and Crowley, B.
(2008)
Detection and molecular characterisation of
plasmidic AmpC b-lactamases in Klebsiella
pneumoniae isolates from a tertiary-care
hospital in Dublin, Ireland.
Clinical Microbiology and Infection, 14 (6).
pp. 616-618.
ISSN 1198-743X
Abstract
This study determined the types of AmpC enzymes produced by Klebsiella pneumoniae isolates resistant to third-generation cephalosporins and the clonality of these isolates. The presence of AmpC enzymes was identified by cephalosporin-cloxacillin synergy tests. Genes encoding AmpC enzymes were characterised by PCR and sequencing. Pulsed-field gel electrophoresis (PFGE) was used to type the isolates. Fifteen K. pneumoniae isolates were positive for bla(AmpC), 13 were positive for bla(ACC-1) and two were positive for bla(DHA-1). Production of the DHA-1 enzyme was inducible. The ampR gene was identified upstream of the bla(DHA-1) gene. PFGE demonstrated the polyclonal origin of the isolates carrying bla(ACC-1).
| Item Type: |
Article
|
| Keywords: |
ACC-1; AmpC b-lactamase; DHA-1; Klebsiella
pneumoniae; resistance; typing; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7478 |
| Identification Number: |
https://doi.org/10.1111/j.1469-0691.2008.01998.x |
| Depositing User: |
Fiona Walsh
|
| Date Deposited: |
28 Sep 2016 15:29 |
| Journal or Publication Title: |
Clinical Microbiology and Infection |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| Funders: |
Health Research Board (HRB) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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