Roche, C. and Boo, T.W. and Walsh, Fiona and Crowley, B. (2008) Detection and molecular characterisation of plasmidic AmpC b-lactamases in Klebsiella pneumoniae isolates from a tertiary-care hospital in Dublin, Ireland. Clinical Microbiology and Infection, 14 (6). pp. 616-618. ISSN 1198-743X

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Abstract

This study determined the types of AmpC enzymes produced by Klebsiella pneumoniae isolates resistant to third-generation cephalosporins and the clonality of these isolates. The presence of AmpC enzymes was identified by cephalosporin-cloxacillin synergy tests. Genes encoding AmpC enzymes were characterised by PCR and sequencing. Pulsed-field gel electrophoresis (PFGE) was used to type the isolates. Fifteen K. pneumoniae isolates were positive for bla(AmpC), 13 were positive for bla(ACC-1) and two were positive for bla(DHA-1). Production of the DHA-1 enzyme was inducible. The ampR gene was identified upstream of the bla(DHA-1) gene. PFGE demonstrated the polyclonal origin of the isolates carrying bla(ACC-1).

Item Type:	Article
Keywords:	ACC-1; AmpC b-lactamase; DHA-1; Klebsiella pneumoniae; resistance; typing;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7478
Identification Number:	https://doi.org/10.1111/j.1469-0691.2008.01998.x
Depositing User:	Fiona Walsh
Date Deposited:	28 Sep 2016 15:29
Journal or Publication Title:	Clinical Microbiology and Infection
Publisher:	Elsevier
Refereed:	Yes
Funders:	Health Research Board (HRB)
URI:	http://www.sciencedirect.com

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