Duffy, Brion and Holliger, E. and Walsh, Fiona
(2014)
Streptomycin use in apple orchards did not increase abundance
of mobile resistance genes.
FEMS Microbiology Letters, 350.
pp. 180-189.
ISSN 0378-1097
Abstract
Streptomycin is used as a first-line defense and tetracycline as a second-line
defense, in the fight against fire blight disease in apple and pear orchards. We
have performed the first study to quantitatively analyze the influence of streptomycin
use in agriculture on the abundance of streptomycin and tetracycline
resistance genes in apple orchards. Flowers, leaves, and soil were collected from
three orchard sites in 2010, 2011, and 2012. Gene abundance distribution was
analyzed using two-way ANOVA and principal component analysis to investigate
relationships between gene abundance data over time and treatment. The
mobile antibiotic resistance genes, strA, strB, tetB, tetM, tetW, and the insertion
sequence IS1133, were detected prior to streptomycin treatment in almost all
samples, indicating the natural presence of these resistance genes in nature. Statistically
significant increases in the resistance gene abundances were occasional,
inconsistent, and not reproducible from one year to the next. We conclude
that the application of streptomycin in these orchards was not associated with
sustained increases in streptomycin or tetracycline resistance gene abundances.
| Item Type: |
Article
|
| Keywords: |
tetracycline; qPCR; relative quantity; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7486 |
| Identification Number: |
https://doi.org/10.1111/1574-6968.12313 |
| Depositing User: |
Fiona Walsh
|
| Date Deposited: |
04 Oct 2016 14:44 |
| Journal or Publication Title: |
FEMS Microbiology Letters |
| Publisher: |
Wiley |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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