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    Proteomic profiling of chronic low-frequency stimulated fast muscle

    Donoghue, Pamela and Doran, Philip and Wynne, Kieran and Federsen, Kasper and Dunn, Michael J. and Ohlendieck, Kay (2007) Proteomic profiling of chronic low-frequency stimulated fast muscle. Proteomics, 7 (18). pp. 3417-3430. ISSN 1615-9853

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    Skeletal muscle fibre transitions occur in many biological processes, in response to alterations in neuromuscular activity, in muscular disorders, during age-induced muscle wasting and in myogenesis. It was therefore of interest to perform a comprehensive proteomic profiling of muscle transformation. Chronic low-frequency stimulation of the rabbit tibialis anterior muscle represents an established model system for studying the response of fast fibres to enhanced neuromuscular activity under conditions of maximum activation. We have conducted a DIGE analysis of unstimulated control specimens versus 14- and 60-day conditioned muscles. A differential expression pattern was observed for 41 protein species with 29 increased and 12 decreased muscle proteins. Identified classes of proteins that are changed during the fast-to-slow transition process belong to the contractile machinery, ion homeostasis, excitation-contraction coupling, capillarization, metabolism and stress response. Results from immunoblotting agreed with the conversion of the metabolic, regulatory and contractile molecular apparatus to support muscle fibres with slower twitch characteristics. Besides confirming established muscle elements as reliable transition markers, this proteomics-based study has established the actin-binding protein cofilin-2 and the endothelial marker transgelin as novel biomarkers for evaluating muscle transformation.

    Item Type: Article
    Keywords: Chronic low-frequency stimulation; DIGE; Fibre transition; Muscle plasticity; Skeletal muscle proteomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7501
    Identification Number:
    Depositing User: Prof. Kay Ohlendieck
    Date Deposited: 11 Oct 2016 15:32
    Journal or Publication Title: Proteomics
    Publisher: Wiley
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Health Research Board (HRB), European Commission
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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