Doran, Philip and Gannon, Joan and O'Connell, Kathleen and Ohlendieck, Kay (2007) Aging skeletal muscle shows a drastic increase in the small heat shock proteins aB-crystallin/HspB5 and cvHsp/HspB7. European Journal of Cell Biology, 86 (10). pp. 629-640. ISSN 0171-9335

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Abstract

Most heat shock proteins operate as molecular chaperones and play a central role in the maintenance of normal cellular function. In skeletal muscle, members of the alpha-crystallin domain-containing family of small heat shock proteins are believed to form a cohort of essential stress proteins. Since alphaB-crystallin (alphaBC/HspB5) and the cardiovascular heat shock protein (cvHsp/HspB7) are both implicated in the molecular response to fibre transformation and muscle wasting, it was of interest to investigate the fate of these stress proteins in young adult versus aged muscle. The age-related loss of skeletal muscle mass and strength, now generally referred to as sarcopenia, is one of the most striking features of the senescent organism. In order to better understand the molecular pathogenesis of age-related muscle wasting, we have performed a two-dimensional gel electrophoretic analysis, immunoblotting and confocal microscopy study of aged rat gastrocnemius muscle. Fluorescent labelling of the electrophoretically separated soluble muscle proteome revealed an overall relatively comparable protein expression pattern of young adult versus aged fibres, but clearly an up-regulation of alphaBC and cvHsp. This was confirmed by immunofluorescence microscopy and immunoblot analysis, which showed a dramatic age-induced increase in these small heat shock proteins. Immunodecoration of other major stress proteins showed that they were not affected or less drastically changed in their expression in aged muscle. These findings indicate that the increase in muscle-specific small heat shock proteins constitutes an essential cellular response to fibre aging and might therefore be a novel therapeutic option to treat sarcopenia of old age.

Item Type:	Article
Keywords:	Aging; alpha-B crystallin; cvHsp; sHsp; Heat shock protein; Sarcopenia;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7504
Identification Number:	https://doi.org/10.1016/j.ejcb.2007.07.003
Depositing User:	Prof. Kay Ohlendieck
Date Deposited:	11 Oct 2016 15:31
Journal or Publication Title:	European Journal of Cell Biology
Publisher:	Elsevier
Refereed:	Yes
Funders:	Science Foundation Ireland (SFI), Health Research Board (HRB)
URI:	http://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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