Glover, Louise and Ohlendieck, Kay (2004) Illustration of Skeletal Muscle Calsequestrin Complex Formation by Cell Overlay and Two-Dimensional Blot Overlay Methodology. Basic and Applied Myology, 14 (4). pp. 231-241. ISSN 1120-9992

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Abstract

Based on the recent finding that a peroxidase-conjugated calsequestrin probe retains its biological binding properties, we have used it in two-dimensional blot and cell overlay procedures. Labeling of nitrocellulose replicas of electrophoretically separated microsomal proteins from predominantly fast versus slow fibres revealed self-aggregation of fast calsequestrin with molecular species of differing isolectric points. Incubation of transverse cryosections showed restricted calsequestrin interactions with elements in the fibre interior, mostly representing calsequestrin itself. This confirms that fast calsequestrin exists in large aggregates under native conditions and demonstrates the usefulness of blot and cell overlay techniques in identifying and locating supramolecular protein assemblies.

Item Type:	Article
Keywords:	blot overlay assay; calsequestrin; cell overlay assay; ryanodine receptor; triadin;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7509
Depositing User:	Prof. Kay Ohlendieck
Date Deposited:	14 Oct 2016 10:23
Journal or Publication Title:	Basic and Applied Myology
Refereed:	Yes
Funders:	Health Research Board (HRB), European Commission
URI:	http://www.bio.unipd.it/bam
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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