O'Reilly, Clare and Pette, Dirk and Ohlendieck, Kay (2003) Increased expression of the nicotinic acetylcholine receptor in stimulated muscle. Biochemical and Biophysical Research Communications, 300 (2). pp. 585-591. ISSN 0006-291X

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Abstract

Chronic low-frequency stimulation has been used as a model for investigating responses of skeletal muscle fibres to enhanced neuromuscular activity under conditions of maximum activation. Fast-to-slow isoform shifting of markers of the sarcoplasmic reticulum and the contractile apparatus demonstrated successful fibre transitions prior to studying the effect of chronic electro-stimulation on the expression of the nicotinic acetylcholine receptor. Comparative immunoblotting revealed that the alpha- and delta-subunits of the receptor were increased in 10-78 day stimulated specimens, while an associated component of the surface utrophin-glycoprotein complex, beta-dystroglycan, was not drastically changed in stimulated fast skeletal muscle. Previous studies have shown that electro-stimulation induces degeneration of fast glycolytic fibres, trans-differentiation leading to fast-to-slow fibre transitions and activation of muscle precursor cells. In analogy, our results indicate a molecular modification of the central functional unit of the post-synaptic muscle surface within existing neuromuscular junctions and/or during remodelling of nerve-muscle contacts.

Item Type:	Article
Keywords:	Low-frequency stimulation; Skeletal muscle; Fast-to-slow transition; Nicotinic acetylcholine receptor; Neuromuscular junction; Calcium-ATPase; Calsequestrin; Agrin; Dystroglycan; Dystrophin;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7513
Identification Number:	https://doi.org/10.1016/S0006-291X(02)02898-X
Depositing User:	Prof. Kay Ohlendieck
Date Deposited:	14 Oct 2016 14:51
Journal or Publication Title:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Refereed:	Yes
Funders:	Health Research Board (HRB), European Commission, Deutsche Forschungsgemeinschaft
URI:	http://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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