O'Brien, Matt and Grogan, Helen and Kavanagh, Kevin
(2014)
Proteomic response of Trichoderma aggressivum
f. europaeum to Agaricus bisporus tissue and
mushroom compost.
Fungal Biology, 118 (9-10).
pp. 785-791.
ISSN 1878-6146
Abstract
A cellular proteomic analysis was performed on Trichoderma aggressivum f. europaeum.
Thirty-four individual protein spots were excised from 2-D electropherograms and analysed
by ESI-Trap Liquid Chromatography Mass Spectrometry (LC/MS). Searches of the
NCBInr and SwissProt protein databases identified functions for 31 of these proteins based
on sequence homology. A differential expression study was performed on the intracellular
fraction of T. aggressivum f. europaeum grown in media containing Agaricus bisporus tissue
and Phase 3 mushroom compost compared to a control medium. Differential expression
was observed for seven proteins, three of which were upregulated in both treatments,
two were down regulated in both treatments and two showed qualitatively different regulation
under the two treatments. No proteins directly relating to fungal cell wall degradation
or other mycoparasitic activity were observed. Functions of differentially produced
intracellular proteins included oxidative stress tolerance, cytoskeletal structure, and cell
longevity. Differential production of these proteins may contribute to the growth of T. aggressivum
in mushroom compost and its virulence toward A. bisporus.
| Item Type: |
Article
|
| Keywords: |
Fungal pathogen; Mushroom production; Mycoparasite; Stress tolerance; Virulence; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7562 |
| Identification Number: |
https://doi.org/10.1016/j.funbio.2014.06.004 |
| Depositing User: |
Dr. Kevin Kavanagh
|
| Date Deposited: |
20 Oct 2016 15:41 |
| Journal or Publication Title: |
Fungal Biology |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| Funders: |
Teagasc |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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