Coyle, Barry and Kavanagh, Kevin and McCann, Malachy and Devereux, Michael and Geraghty, Majella
(2003)
Mode of anti-fungal activity of 1,10-phenanthroline and its Cu(II), Mn(II)
and Ag(I) complexes.
Biometals, 16.
pp. 321-329.
ISSN 0966-0844
Abstract
The mode of action of the anti-fungal compounds, 1,10-phenanthroline (phen), [Cu(phen)2(mal)] · 2H2O,
[Mn(phen)2(mal)] · 2H2O and [Ag2(phen)3(mal)] · 2H2O (malH2 = malonic acid), was examined using the pathogenic
yeast Candida albicans. The compounds have minimum inhibitory concentrations (MIC’s) in the range
1.25–5.0 μg cm−3 and at a concentration of 10 μg cm−3 display some fungicidal activity. Yeast cells exposed to
these drugs show a diminished ability to reduce 2,3,5-triphenyltetrazolium chloride (TTC), indicating a reduction
in respiratory function. Treating exponential and stationary phase yeast cells with phen and the Cu(II) and Mn(II)
complexes causes a dramatic increase in oxygen consumption. All of the drugs promote reductions in the levels of
cytochromes b and c in the cells, whilst the Ag(I) complex also lowers the amount of cytochrome aa3. Cells treated
with phen and the Cu(II) and Ag(I) species show reduced levels of ergosterol whilst the Mn(II) complex induces an
increase in the sterol concentration. The general conclusion is that the drugs damage mitochondrial function and
uncouple respiration. That the drugs are not uniformly active suggests their bioactivity has a degree of metal-ion
dependency. Phen and metal-phen complexes represent a novel set of highly active anti-fungal agents whose mode
of action is significantly different to that of the polyene and azole prescription drugs.
| Item Type: |
Article
|
| Keywords: |
anti-fungal; Candida; fungicidal; fungistatic; metal complexes; 1,10-phenanthroline; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
7591 |
| Identification Number: |
https://doi.org/10.1023/A:1020695923788 |
| Depositing User: |
Dr. Kevin Kavanagh
|
| Date Deposited: |
02 Nov 2016 16:46 |
| Journal or Publication Title: |
Biometals |
| Publisher: |
Kluwer Academic Publishers |
| Refereed: |
Yes |
| Funders: |
Enterprise Ireland (EI) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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