Browne, Niall and Surlis, Carla and Maher, Amie and Gallagher, Clair and Carolan, James C. and Clynes, Martin and Kavanagh, Kevin (2015) Prolonged pre-incubation increases the susceptibility of Galleria mellonella larvae to bacterial and fungal infection. Virulence, 6 (5). pp. 458-465. ISSN 2150-5594

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Abstract

Galleria mellonella larvae are widely used for assessing the virulence of microbial pathogens and for measuring the in vivo activity of antimicrobial agents and produce results comparable to those that can be obtained using mammals. The aim of the work described here was to ascertain the effect of pre-incubation at 15°C for 1, 3, 6 or 10 weeks on the susceptibility of larvae to infection with Candida albicans and Staphylococcus aureus. Larvae infected with C. albicans after 1 week pre-incubation at 15°C showed 73.3 § 3.3% survival at 24 hours post-infection while those infected after 10 weeks pre-incubation showed 30 § 3.3% survival (P < 0.01). Larvae infected with S. aureus after 1 week preincubation showed 65.5 § 3.3% survival after 24 hours while those infected after 10 weeks pre-incubation showed 13.3 § 3.3% (P < 0.001). Analysis of the haemocyte density in larvae pre-incubated for 3–10 weeks showed a reduction in haemocytes over time but a proportionate increase in the density of granular haemocytes in the population as determined by FACS analysis. Proteomic analysis revealed decreased abundance of proteins associated with metabolic pathways (e.g. malate dehydrogenase, fructose-1,6-bisphosphatase, glyceraldehyde-3-phosphate dehydrogenase) and prophenoloxidase. G. mellonella larvae are a useful in vivo model system but the duration of the pre-incubation stage significantly affects their susceptibility to microbial pathogens possibly as a result of altered metabolism.

Item Type:	Article
Keywords:	age; Galleria; immune response; immunity; insect; larvae; mini-model;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Institute of Immunology
Item ID:	8173
Identification Number:	https://doi.org/10.1080/21505594.2015.1021540
Depositing User:	James Carolan
Date Deposited:	25 Apr 2017 16:00
Journal or Publication Title:	Virulence
Publisher:	Landes Bioscience
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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