Kelly-Rogers, Jane and Madrigal-Estebas, Laura and O'Connor, Tony and Doherty, Derek G. (2006) Activation-Induced Expression of CD56 by T Cells Is Associated With a Reprogramming of Cytolytic Activity and Cytokine Secretion Profile In Vitro. Human Immunology, 67. pp. 863-873.

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Abstract

A subset of human T lymphocytes expresses the natural killer (NK) cell-associated receptor CD56 and is capable of major histocompatibility complex (MHC)-unrestricted cytotoxicity against a variety of autologous and allogeneic tumor cells. CD56+T cells have shown potential for immunotherapy as antitumor cytotoxic effectors, but their capacity to control adaptive immune responses via cytokine secretion is unclear. We have examined the inducibility of CD56+T cells from human blood in vitro and compared the kinetics of Th1, Th2, and regulatory cytokine secretion by CD56+T cells with those of conventional CD56¯ T cells. CD56 was induced on CD8+ and CD4¯CD8¯ T cells by CD3/T-cell receptor (TCR)- mediated activation, particularly when grown in the presence of interleukin (IL)-2. Activation induced CD56+ T cells proliferated less vigorously but displayed enhanced natural cytotoxicity compared with CD56¯ T cells. CD56+ T cells released interferon-y )IFN-y) and interleukin-13(IL-13), but not IL-10, upon TCR stimulation. Flow cytometric analysis demonstrated that, compared with CD56¯ T cells, elevated proportions of CD56+ T cells expressed IFN-y, IL-4, and IL-13 within hours of activation. These acquired cytolytic and cytokine secretion activities of CD56+ T cells make them potential targets for immunotherapy for infectious and immune-mediated disease.

Item Type:	Article
Keywords:	CD56+T cells: natural killer cells: cytokines: cytotoxicity: kinetics:
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	896
Depositing User:	Dr. Derek Doherty
Date Deposited:	13 Feb 2008
Journal or Publication Title:	Human Immunology
Publisher:	Elsevier
Refereed:	Yes
URI:	http://www.sciencedirect.com/science
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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